Purpose: Thick and thin blood films stained with Giemsa hematological stains permit the detection of blood parasites including malarial parasites, trypanosomes, and microfilariae. Place the blood film on a tray or drying rack. The highest yield of peripheral parasites occurs during or soon after a _____; however, smears should not be delayed while awaiting _____. Remove and let air dry. Fix air-dried film in absolute methanol by dipping the film briefly (two dips) in a Coplin jar containing absolute methanol. thin or thick blood smear. Kenya Medical Research Institute (KEMRI) [22] [23]. Flush with tap water and leave to dry For the best results, the smear should be stained with a 3% Giemsa solution (pH of 7.2) for 30 - 45 minutes. A blood smear reveals information about the number and shape of blood cells in the body. Doctors use thick and thin blood smears to determine whether you have malaria. diagnosis of malaria should be supported by the identification of the parasites on a _____ 12-24. smears should be obtained _____ hours apart. Add the buffer. Aim of blood smear • Blood films are usually examined to investigate hematological problems (disorders of the blood) and, occasionally, to look for parasites within the blood such as malaria and filaria. However, they do not permit an optimal review of parasite morphology. A blood smear, also referred to as a peripheral smear for morphology, is an important test for evaluating blood-related problems, such as those in red blood cells, white blood cells, or platelets.It has a wide range of uses, including distinguishing viral infections from bacterial infections, evaluating anemia, looking for causes of jaundice, and even diagnosing malaria. Thin blood is the opposite of thick blood, which can increase the risk of blood clots and complications, such as stroke. The method uses Artificial Neural Net- work (ANN) to test for the presence of plasmodium parasites in thin blood smear images. Air dry the thin film, fix it with methyl alcohol, and immediately stain it. the purpose and criteria for blood smear examination in a variety of circumstances that are encountered in everyday laboratory hematology practice. Description of the proposed method and database is described in Section II. 30×) than in an equal area of a thin smear. 2. Thick smears should be left in buffer for 5 minutes. the examination of a blood smear when such protocols indicate that it is necessary. All you wanted to know about the blood smear test or the peripheral smear test, learn the Purpose, procedure and what the results mean. A frog blood smear is a thin layer of frog's blood that has been carefully spread on a glass microscope slide. Purpose and Criteria for Blood Smear Scan, Blood Smear Examination, and Blood Smear Review.pdf Available via license: CC BY-NC 3.0 Content may be subject to copyright. Peripheral blood smear test is ordered as part of a general health exam to help diagnose many illnesses. A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. Leishman stain, also known as Leishman's stain, is used in microscopy for staining blood smears.It is generally used to differentiate between and identify white blood cells, malaria parasites, and trypanosomas.It is based on a methanolic mixture of "polychromed" methylene blue (i.e. This method produces a gradual decrease in thickness of the blood from thick to thin ends with the smear terminating in a feathered edge approximately 2 mm long. Exam Overview. On a clean dry microscopic glass slide, make a thin film of the specimen (blood) and leave to air dry. For Thin blood smear . Note: As alternates to this 45-60 minutes in 2.5% Giemsa stain, the smears could be stained for shorter times in more concentrated stains. The Procedure of Giemsa staining varies as per the purpose of staining that means whether the staining is done for the examination of Blood cells or to find the Parasites in the blood smear and accordingly the Blood smears are prepared as Thin Blood films or Thick blood films. Ignou student. Results . You can make perfect blood smear by using 10 micro L of blood on a slide The smear allows the various components of the blood to be viewed through the microscope. It helps diagnose if red blood cells, white blood cells and platelets are normal in appearance and number. 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