Mice were either picked up by the tail or guided into a tunnel, then transferred to the testing arena. Tunnel handling improves the welfare of mice undergoing repeated procedures Thursday 10 September 2020 New research published in Scientific Reports shows the animal welfare benefits of picking up laboratory mice via a tunnel instead of by the tail persist even … They observed that BALB/c mice found restraint more stressful than handling alone, and showed evidence of a stronger aversion to IP injections than isoflurane anaesthesia. These positive behavioural effects persisted even after repeated aversive procedures. The FAQs address common misconceptions. Improving handling techniques for laboratory mice helps reduce stress and anxiety, according to a study published today in the journal ‘Nature Methods’. Scientific Reports 10: e14562. Need vole removal in your hometown? The researchers showed that mice of all strains and sex developed a consistent voluntary interaction in the ninth daily handling session among all the three handling methods – the tail, home cage tunnel, or "cupped" on open hand . Picking up mice by the tail can compromise their welfare and affect scientific outcomes. If cupped, open hands or clear tunnels are used to pick up the mice, they will show normal curiosity and behavior once placed in the test environment. For 96” OD and larger hand tunnels, Bradshaw is the utility industry leader in using the NATM/SEM (sprayed shotcrete) hand tunneling method. Read answers to frequently asked questions about tunnel and cup handling. To test discrimination between two different scents, the mice were then placed near a different urine stimulus. As handling mice by a tunnel requires minimal physical contact with the animal, this is likely to be another advantage for less experienced handlers. New research published in Scientific Reports shows the animal welfare benefits of picking up laboratory mice via a tunnel instead of by the tail persist even after repeated restraint, injection and anaesthesia. According to the MGS, tattooing was not more painful than ear tagging but caused significant tail inflammation and more agitation and anxiety. Their work adds to the substantial evidence supporting tunnel handling as a way to improve the welfare of mice and reduce handling-related stress, even when experimental protocols require the use of more aversive procedures. They were also less likely to defecate during handling and behavioural testing than their tail-handled counterparts. These non-aversive methods also make handling easier for researchers and animal care staff, and enhance mouse performance in behavioural tests. However, two alternative methods for picking up laboratory mice have been investigated and validated in recent years [7–9]; ‘tunnel’ handling, that involves guiding mice into a tunnel before being lifted (thus avoiding direct contact), and ‘cup’ handling, where mice are scooped up and lifted with closed or open hands and allowed to move freely without direct physical restraint (video tutorials of … Traditionally mice have been picked up uh via their tails. The original research by Professor Hurst was funded by the BBSRC and the NC3Rs. Mice can then be encouraged to climb into the beaker (Figures 31.3 and 31.4). The research used ... measures which showed that the tail handled mice were more anxious, depressed and chronically stressed than the tunnel handled mice. Crucially, the authors found that tunnel handling alleviated the negative behavioural effects of the aversive procedures tested, unlike traditional tail handling. The investment in training should be outweighed by the benefits observed with more reliable behavioural and physiological responses in the mice. Hear from champions based at facilities around the UK who have successfully implemented non-aversive mouse handling methods. Tunnel handling. While mice may appear small and cute, they can create big problems in your yard as they build tunnels and chew on your flowers, vegetables and shrubs. Laboratory mice are routinely handled for husbandry and scientific procedures. Non-aversive methods do not add time to husbandry or procedures provided staff are adequately trained. It won the 2010 NC3Rs 3Rs Prize. doi: 10.1038/s41598-020-71476-y. A study funded by the NC3Rs explored how handling methods influenced mice’s behavior during cognitive tasks. Using a computer mouse may not seem dangerous, but potentially wrist-driven actions repeated thousands and thousands of times have the potential to cause carpal tunnel syndrome. Download short video clips for use in in-house training. Here we provide practical tips on non-aversive handling, including a webinar, a video tutorial, and the underpinning evidence base. By contrast, mice picked up in a tunnel explored their environment readily, showed a strong interest in the new stimulus, and a clear effect of becoming familiar with it in the consecutive sessions. Mice quickly habituate to tunnel handling and can subsequently be restrained by the scruff or tail base for procedures or health and welfare assessments without negating the … Hand tunneling is typically performed by tunnel miners using pneumatic hand tools to excavate the soils and then installing wood and/or steel supports for tunnels 42” OD or larger. Moreover, restraining the tunnel- and cupping-handled mice by neck skin “scruffing” did not alter their willingness to interact with the handler. Research has shown that picking up mice by the tail induces aversion and high anxiety and generally should be avoided. You can then use these tunnels to transfer the mice between cages for things such as cleaning out. The techniques identified in the study should lead to further improvements in the welfare of the mice. If you are the type to use a computer heavily, you face both a high risk for … For example, they may be restrained and injected many times over the duration of a study. Using a tunnel or cupped hand to pick up mice causes less anxiety than traditional tail handling. Handling stress is a well-recognised source of variation in animal studies that can also compromise the welfare of research animals. A reel may also serve as means of transfer, as mice like to crawl into the dark tube or climb onto the device. 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